Discrimination between pathogenic and non-pathogenic E. coli strains by means of Raman microspectroscopy
نویسندگان
چکیده
منابع مشابه
DNA microarray for discrimination between pathogenic 0157:H7 EDL933 and non-pathogenic Escherichia coli strains.
The primary technique currently used to detect biological agents is based on immunoassays. Although sensitive and specific, currently employed immunoassays generally rely on the detection of a single epitope, and therefore often cannot discriminate subtle strain-specific differences. Since DNA microarrays can hybridize hundreds to thousands of genomic targets simultaneously and do not rely on p...
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The persistence of Shiga-like toxin producing E. coli (STEC) strains in the agricultural soil creates serious threat to human health through fresh vegetables growing on them. However, the survival of STEC strains in Indian tropical soils is not yet understood thoroughly. Additionally how the survival of STEC strain in soil diverges with non-pathogenic and genetically modified E. coli strains is...
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The genome sequences of enterohaemorrhagic E. coli O157:H7 strains show multiple open-reading frames with collagen-like sequences that are absent from the common laboratory strain K-12. These putative collagens are included in prophages embedded in O157:H7 genomes. These prophages carry numerous genes related to strain virulence and have been shown to be inducible and capable of disseminating v...
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Adherent-invasive Escherichia coli (AIEC) pathovar strains, which are associated with Crohn's disease, share many genetic and phenotypic features with extraintestinal pathogenic E. coli (ExPEC) strains, but little is known about the level of genetic similarity between the two pathovars. We aimed to determine the frequency of strains with the "AIEC phenotype" among a collection of ExPEC strains ...
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Objective: Main aim of the present study was to determine differential effects of temperature and pH on the sensitivity/resistance of bacteria against ciprofloxacin and chloramphenicol. Methods: Both the bacterial strains were subjected to MIC determination. These were subjected to the varying values of environmental stresses including temperature and pH. The growth was monitored using spectrop...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2020
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-020-02957-2